Coding
Cel5A

Part:BBa_K1350007

Designed by: Yiting Luo   Group: iGEM14_SZU-China   (2014-09-28)

Cel5A, endoglucanase(BBa_K805011 Improved)


The endoglucanase genes cel5A can hydrolyze β-1,4glucosan randomly and act on long cellulose chains. The main product is oligomeric glucose. It can digest glucosan to produce glucose monomer in high efficiency with synergy with β-1,4glucosidase.It shows high ability to hydrolyze the crystal cellulose but relative lower enzyme activity towards carboxymethyl cellulose, barley β-glucan, and PASC.

Improved endoglucanase Cel5A

BBa_K805011[1] given by iGEM12_HUST-China had BamH I site. The site is cleared by introducing a silent mutation. GGATCC (874-879) has been changed to GGGTCC. A codon GGA(Gly) is changed to GGG(Gly). Because of the degeneracy of codon,the different sequence can encode the same enzyme.This silent mutation makes the part can be compatible with RFC[21].



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 307
    Illegal NgoMIV site found at 763
    Illegal AgeI site found at 1027
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1024


[edit]
Categories
Parameters
n/aCel5A, endoglucanase(BBa_K805011 Improved)